Structural Biology Research Associate

5 days ago

Vancouver, British Columbia, Canada UBC Full time
Job Summary

This position is required to support day-to-day macromolecular sample preparation for structural biology as well as infrastructure operation and eventual user training in the High Resolution Macromolecular EM Facility at UBC.

The incumbent will be responsible for undertaking standard molecular biology and protein overexpression, purification procedures to create high yield, high purity proteins for structural biology applications.

Key responsibilities include:

  • Operating and maintaining a 120 keV Talos TEM instrument in both room temperature and cryo modes.
  • Handling and clipping cryoEM grids in preparation for imaging, as well as handling of cryoEM grids for transfer to multi-sample autoloader capsules.
  • Overseeing and maintaining a VitroBot and other vitrification equipment.
  • Working with lab members and users to troubleshoot problems and answer queries.
  • Overseeing and performing logistical tasks including organizational tasks, equipment maintenance and oversight, user session scheduling, placing orders, handling liquid nitrogen, and tracking usage.
  • Assisting with lab and facility communication including website population and design and creation or update of standardized operational procedure documentation.

The successful candidate will have a strong background in molecular biology and protein purification, as well as experience with electron microscopy and cryoEM. They will also have excellent communication and interpersonal skills, with the ability to work effectively in a team environment.

Key Qualifications

  • Completion of a university degree in a relevant discipline or technical program and a minimum four years of related experience or an equivalent combination of education and experience.
  • Strong background in molecular biology and protein purification.
  • Experience with electron microscopy and cryoEM.
  • Excellent communication and interpersonal skills.

Preferred Qualifications

  • Undergraduate degree in a relevant discipline, or graduation from a technical college or institute.
  • MSc in a relevant discipline.
  • Prior expertise of sample preparation for structural biology including membrane proteins.
  • Knowledge of the general work flow of single particle cryoEM.

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