Research Technician Ii/iii/iv

3 weeks ago

Toronto, Canada University Health Network Full time

**Job Title: Research Technician II/III/IV (Permanent Full-Time)**

**Job Posting #: 926399**

**Union**:NON-UNION

**Site**:Princess Margaret Cancer Research Tower

**Department**:Research**
**Reports to**:Principal Investigators**
**Hours**:37.5 hours per week

**Salary: $57,018 - 92,625: To commensurate with experience and consistent with UHN compensation policy**

**Status**:Permanent Full-time

**Posted Date: November 8, 2023**

**Closing Date: December 6, 2023**

The University Health Network, where “above all else the needs of patients come first”, encompasses Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute and the Michener Institute of Education. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of ground breaking firsts and a purpose of “Transforming lives and communities through excellence in care, discovery and learning”, the University Health Network (UHN), Canada’s largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.

**Position Summary**

We are searching for a skilled Research Technician to join our fast-paced research laboratories focused on the post-transcriptional circuitries governing normal and malignant hematopoietic stem cells, and ubiquitin and ubiquitin-like modifier protein populations in normal and various disease states. The individual will be required to work closely with students and staff to perform in vivo mouse studies and wet-lab experiments under the direction of senior staff, and principal investigators Dr. Kristin Hope and Dr. Brian Raught.

**Duties**
- Perform cell line and primary patient cell culture assays, lentiviral transductions, drug treatment studies with pro-hematopoietic or anti-leukemic compounds
- Carry out in vivo studies in immunodeficient mice, including xenotransplantations, animal monitoring, drug administration, and tissue analysis
- Perform multi-parameter flow cytometry and cell sorting
- Prepare samples for next generation omics technologies and high throughput screens
- Carry out standard molecular and biochemical experiments
- Assist in developing in vivo and in vitro protocols
- Perform additional tasks as required, including but not limited to molecular cloning, lentivirus preparation, cell culture, and whole blood processing
- Supervise and train undergraduate or graduate students
- Maintain laboratory equipment and inventory

**Qualifications**
- At minimum, completion of a B.Sc. in a scientific discipline (Biochemistry, Molecular
- Biology, or a related field)
- At least three (3) years of technical experience in an academic laboratory
- Must have experience with mouse models and rodent handling, standard mammalian cell culture, and basic wet lab techniques (bacterial culture, DNA/RNA/protein isolation, agarose/acrylamide gels, etc)
- Experience with multi-parameter flow cytometry, sample library preparation, and next-generation omics considered an asset
- Experience with molecular approaches including CRISPR and high throughput screen design/implementation considered an asset
- Collaborative mindset, able to follow instructions, and willing to work independently but also closely with other members of the team
- A willingness to learn new techniques and technologies
- Great problem-solving and critical thinking skills
- Excellent organizational and time management skills
- Excellent competency in written and verbal communication
- Excellent computer skills in MS Office. Experience with R, Python, FlowJo, and GraphPad an asset

**Why join UHN?**

In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.
- Competitive offer packages
- Close access to Transit and UHN shuttle service
- A flexible work environment
- Opportunities for development and promotions within a large organization
- Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

Vaccines (COVID and others) are a requirement of the job unless you have an exemption on a medical ground pursuant to the Ontario Human Rights Code.

UHN is a respectful, caring, and in

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