Research Technician

2 weeks ago

Ottawa, Canada OHRI Full time

**Responsibilities include**:

- Managing mouse strains, mouse breeding, genotyping, and record keeping.
- Assist members of the lab performing mouse experiments.
- Sterile processing of umbilical cord blood, peripheral blood, and bone marrow samples
- Assist in cell culture of various cell lines including human pluripotent stem cells.
- Manage inventory of cell lines.
- Perform defined molecular biology (eg, qPCR, cloning) and biochemistry (eg, Western blots) experiments in collaboration with lab members.
- Present research progress in lab meetings.
- Assist in administrative tasks.
- Maintain updated records electronically for all procedures and tasks.
- Quality test new reagents, including media or substrates.
- Perform mycoplasma DNA testing by PCR for cell lines.
- Ship cells to collaborating labs.
- Assist other staff in administrative and financial tasks.
- Assist other staff and trainees in research projects as needed, including CRISPR gene editing or stem cell differentiation/reprogramming protocols.

**Basic Requirements**:

- A minimum of a three-year college diploma, or BSc in biological sciences combined with experience in cell culture, cell-based and quality control assays
- 1-5 years of experience working in a research laboratory
- Possess excellent sterile technique and experience in mammalian cell culture.
- Experience in basic molecular biology techniques: DNA and RNA extractions, molecular cloning, PCR, gel electrophoresis.
- Outstanding organizational skills and attention to detail.
- Ability to manage a large number of samples.
- Excellent record keeping and ability to use an electronic database.
- Time management skills and flexibility with respect to multitasking.
- Ability to work both independently and in teams.
- Excellent interpersonal and communication skills.
- Ability to work part time on weekends on a regular or semi-regular basis.

**Preferred Qualification**:

- Experience in cell culture.
- Experience in CRISPR gene editing.
- Experience in digital droplet PCR and/or qPCR.
- Experience in quality control.

**Contract Details**:
This is a 2-year full-time contract with the expectation of renewal.

Salary Min: $21.278/hr - Max:$30.747/hr

**Comments to Applicant**:
**Contact Info**:
Name: Erin Hicke

Program: Regenerative Medicine Program

Ottawa Hospital Research Institute

Address: 501 Smyth Road, Ottawa, ON K1H 8L6
- The Ottawa Hospital Research Institute is committed to building and sustaining a welcoming and supportive culture that promotes equity, diversity and inclusion for trainees, researchers, and staff. We are devoted to identifying and removing barriers that limit equitable access to opportunities within our organization. The Ottawa Hospital Research Institute strongly supports initiatives that encourage the participation of all groups, in particular the four groups defined in the Employment Equity Act (women, Indigenous peoples, persons with disabilities and members of visible minorities)._
- We thank all applicants for their interest. However, only those invited for an interview will be contacted. The Ottawa Hospital Research Institute is an equal-opportunity employer. Upon request, accommodations due to a disability are available throughout the selection process._

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