Research Technician Ii

3 weeks ago


Toronto, Canada University Health Network Full time

**JOB POSTING#91891**

Position: Research Technician II

Site: Toronto General Hospital Research Institute

Department: Research

Reports to: Manager

Compensation: $28.25 -$35.31 per hour

Hours: 37.5 HOURS PER WEEK

Status: Temporary Full-time (1 year with possibility of renewal)

**University Health Network (UHN)** is looking for an experienced professional to fill the key role of **Research Technician II **at the Toronto General Hospital Research Institute.
- Transforming lives and communities through excellence in care, discovery and learning._

The University Health Network, where “above all else the needs of patients come first”, encompasses **Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, Toronto General Hospital, Toronto Western Hospital, and the Michener Institute of Education at UHN**. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of groundbreaking firsts and a purpose of “Transforming lives and communities through excellence in care, discovery and learning”, the University Health Network (UHN), Canada’s largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.

University Health Network (UHN) is a research hospital affiliated with the University of Toronto and a member of the Toronto Academic Health Science Network. The scope of research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. Research across UHN's five research institutes spans the full spectrum of diseases and disciplines, including cancer, cardiovascular sciences, transplantation, neural and sensory sciences, musculoskeletal health, rehabilitation sciences, and community and population health. Find out about our purpose, values and principles here.

Seeking an individual to join the lab of Dr. Sarah Crome as a Research Technician within the Ajmera Transplant Centre at Toronto General Hospital Research Institute and the Department of Immunology at the University of Toronto. Dr. Crome’s research program is focused on identifying mechanisms of immune tolerance and developing immunotherapies for transplantation and autoimmunity. There is a particular interest in understanding the role of specialized tissue resident immune cells in immune tolerance and developing ways to harness these lymphocytes in novel therapies. The research team is comprised of scientifically enthusiastic individuals who value diversity and supports each other’s individual goals.

**Key responsibilities will include the following**:

- Performing experiments under the supervision of the principle investigator, which will include cell culture, flow cytometry and animal models.
- Processing of patient samples including human tissues and blood.
- Preparing lentiviral vectors, maintaining molecular biology stocks and performing cloning experiments
- Responsible for coordinating and performing assigned routine standard testing.
- Responsible for ensuring reagent supplies are replenished.
- Ensures that cleaning procedures, calibration and/or validation of equipment is performed regularly.
- Aid in generating SOPs, maintaining accurate records of procedures, animals, and inventories.
- Attending and participating in laboratory meetings.
- Training or assisting graduate students in laboratory techniques as appropriate.
- Participating in data analysis

**Skills and Qualifications**:

- Minimum B.Sc plus experience in a research setting (3+ years). M.Sc or additional education in immunology, biological sciences or other related discipline an asset.
- Experience in following techniques is an asset: flow cytometry, tissue staining, humanized mouse models, processing patient samples (tissue and blood), working with lentivirus or other viral vectors, molecular cloning and good manufacturing practices.
- Self-motivated, reliable, detail-oriented, excellent organizational skills.
- Demonstrated ability to work independently and willingness to work closely with other members of the team.
- Computer skills: Microsoft office proficiency, experience with Graphpad prism,Adobe illustrator, Flowjo or R an asset.
- Demonstrated strong written and verbal communications skills.

Vaccines (COVID-19 and others) are a requirement of the job unless you have an exemption on a medical ground pursuant to the Ontario Human Rights Code.

**Posting Date: January 19, 2023 Closing Date: February 19, 2023**

For current UHN employees, only those who have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, and possess all the required experience and qu



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