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Canada-Principal Scientist I

4 weeks ago

Toronto, Canada TalentBurst, Inc. Full time
Principal Scientist I (Scientific)
Toronto, ON
12 Months

Department Description:
The Vaccine Drug Substance Development (VDSD) within the Global Bioprocess Development (GBD) department actively supports process development of Client vaccine candidates utilizing techniques such as molecular engineering, viral cell culture, bacterial fermentation, molecular biology and supportive analytical techniques. Client vaccine candidate are typically recombinant proteins produced in bacterial expression systems or attenuated viruses / glycosylated proteins produced in mammalian expressions system. In VDSD, we develop and scale up upstream and downstream processes for new vaccines up to 200L scale. Working within one of the most highly regulated industries, the candidate will have the opportunity to develop the ability to lead and function creatively within a team, while maintaining attention to strict compliance. The candidate will acquire knowledge of industry constraints and regulations.

Main responsibilities:

The goal of this position is to contribute to generation of pre-master constructs and development of scalable GMP-compliant upstream processes. The candidate will have the opportunity to gain experience with sophisticated laboratory instruments and analytical methods.

The main responsibilities include:
  • Interphase with Global Antigen Design team to screen new vaccine candidate, design and improve various constructs through molecular engineering techniques and perform genetic stability testing
  • Collaborate with Upstream Scientists and lead a team of Technologists to plan and execute upstream process optimization and scale-up studies using technologies such as AMBR high-throughput bioreactor system, small and pilot scale bioreactors
  • Perform in-house or coordinate supportive analytical testing including viral titration assays, FACS, SDS-PAGE, Western blot, HPLC, protein assays, and others
  • Perform statistical data analysis, prepare technical reports and stakeholder presentations
  • Lead and/or contribute to characterization studies using Design of Experiments methodology
  • Contribute to tech transfer of processes to Global Clinical manufacturing or Manufacturing Sciences and Technology
  • Contribute to Sanofi's digital initiatives (eg. AI)
  • Ensure compliance with Sanofi's GxP quality, documentation requirements and HSE policies.

The successful candidate will have a well-developed scientific aptitude and an enthusiasm for hands on laboratory work. There will be the opportunity to gain experience with cutting-edge bioprocess technologies associated with upstream bioprocessing and analysis, gain expertise in developing a scalable GMP-compliant process and make a real contribution to a New Vaccine project.

Experience / Technical skills:
  • Hands-on experience in molecular engineering tools (molecular cloning, nucleic acid analysis, and protein expression)
  • Demonstrated experience with mammalian cell culture (eg. SF9, CHO, Vero)
  • Experience in analytical techniques such as virus titration assays, FACS, protein assays, SDS-PAGE, Western blot, HPLC, and others
  • Knowledge and experience with bioprocessing methods and process scale-up
  • Mandatory Computer skills: Word, Excel, Powerpoint
  • Expertise in Statistical analysis and software (JMP, GraphPad Prism etc) would be an asset


" Soft skills:
" Leadership skills
" Superior organizational skills
" Demonstrated accountability and motivation
" Strong attention to detail and analytical skills
" Excellent communication and interpersonal skills
" Desire to work within a team environment

Education:
Recommended PhD with 1+ years' experience or MSc with 3+ years' experience in a Biochemical Engineering / Life Sciences / Biology / Immunology / Biochemistry disciplines

Languages: English proficiency

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