Lab Research Project Coordinator

2 weeks ago

Toronto, Canada SickKids Full time

**About SickKids**:
Dedicated exclusively to children and their families, The Hospital for Sick Children (SickKids) is one of the largest and most respected paediatric healthcare centres in the world. As innovators in child health, we lead and partner to improve the health of children through the integration of healthcare, leading-edge research and education. Our reputation would not have been built - nor could it be maintained - without the skills, knowledge and experience of the extraordinary people who come to work here every day. SickKids is committed to ongoing learning and development, and features a caring and supportive work environment that combines exceptionally high standards of practice.

When you join SickKids, you become part of our community. We share a commitment and determination to fulfill our vision of _Healthier Children. A Better World_.

Don’t miss out on the opportunity to work alongside the world’s best in paediatric healthcare.

**Position Description**:

- The Howell Lab at The Hospital for SickChildren

**Job responsibilities**:
**_
Experimental_**
- Provide bacterial genetics support to lab. Clone plasmids and develop new strains for projects.
- Develop fluorescently tagged constructs for confocal imaging.
- Localize fluorescently tagged proteins via confocal and/or TEM microscopy.
- Determine protein location via subcellular fractionation and Western blot analysis.
- Express and purify protein from bacterial, yeast, and mammalian cell lines.
- Aid in the development of new expression and purification protocols.
- Develop new assays for the characterization of protein products.
- Provide technical assistance for a variety of diversified basic laboratory techniques.

**_
Administrative_**
- Assist in developing, devising, or improving existing methods, practices, and procedures.
- Order and maintain inventories of supplies, chemical stocks, software, and equipment.
- Coordinate experiments between collaborating labs.
- Support and/or train other lab personnel.
- Maintain and oversee laboratory equipment.
- Uphold safety practices and act as safety representative for the lab.

**Here¿s what you¿ll need**:

- Master¿s degree with 3 years¿ working experience, or a BSc degree with 5 years working experience in Microbiology, Molecular biology, Biochemistry, or Structural Biology.
- Experience with bacterial genetics (PCR, gene cloning, plasmid design, and site-directed mutagenesis)
- Experience with CRISPR is an asset
- Experience with confocal and/or TEM microscopy as well as fluorescent tagging techniques is an asset.
- Experience with standard biochemical, microbiological, and molecular biological lab techniques.
- Experience with recombinant protein expression and purification from bacterial and mammalian hosts.
- Demonstrated competency in written and verbal communications.
- Ability to work independently as well as part of a team.
- Committed to continuous learning and improvement.
- High attention to detail.
- Excellent organizational and project management skills.
- **Employment type**
- Lab Coordinator- Full Time position- Initial contract 1 year (with possibility of renewal)- Start date April 2024**How to Apply**-
**Our Commitment to Diversity**:
The children and families we care for are from a variety of communities, and so are our employees. We welcome and encourage everyone to join our organization and be part of our team.

Thank you for your interest in joining SickKids. Only those applicants selected for an interview will be contacted. In accordance with our Hospital policy, employment at SickKids is conditional upon the Hospital’s receipt of all necessary documentation.

**How to Apply

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