Sessional Lecturer Bch377h Biochemistry Lab I

1 week ago


Toronto, Canada University of Toronto Full time

**Course Number and Title**: BCH 377 H - Biochemistry Lab I

**Course Description**:
This course is designed to build upon the laboratory material introduced in BCH242Y, and to provide a foundation for more advanced methods utilized in the subsequent biochemistry lab courses which are taken by BCH and IMM Specialists. The topics covered include: i) Properties of buffers; 2) Enzyme properties and kinetics (Michaelis-Menten analyses); 3) Protein purification and analysis, including chromatography, spectrophotometric protein assays, enzyme assays, SDS-PAGE and mass spectrometry analysis; 4) Isolation of genomic DNA, PCR analysis, and agarose gel electrophoresis; 5) Methods to study protein unfolding (chemical and thermal denaturation), and protein expression, stability and degradation (western blotting and enzyme assays).

**Estimated Course Enrollment**: 30

**TA Support**: Yes - Size of Appointment: 175 hours

**Class Schedule**: Tues (9am-1pm), Lectures Thurs (11am-12pm) (Fall Session)

**Sessional Appointment Date**: September - December 2023 (Fall Session)

**Salary**: $9,182.42 (inclusive of vacation pay)

**Minimum Qualifications**: Applicant must have convocated with a Ph.D to be given consideration for this position.

**Preferred Qualification**: Same as minimum qualifications.

1 King’s College Circle, Toronto. M5S 1A8.


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