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Laboratory Technician

4 months ago

Toronto, Canada University of Toronto Full time

**Date Posted**:03/15/2023
**Req ID**: 30281
**Faculty/Division**: Faculty of Arts & Science
**Department**: Cell and Systems Biology
**Campus**: St. George (Downtown Toronto)
**Position Number**: 00001866

**Description**:
**About us**:
The Faculty of Arts & Science is the heart of Canada’s leading university and one of the most comprehensive and diverse academic divisions in the world. The strength of Arts & Science derives from our combined teaching and research excellence in the humanities, sciences and social sciences across 29 departments, seven colleges and 46 interdisciplinary centres, institutes and programs.

We can only realize our mission with the dedication and excellence of engaged staff and faculty. The diversity of opportunities and perspectives within the Faculty reflect the local and global landscape and the need for curiosity, innovative thinking and collaboration. At Arts & Science, we take pride in our legacy of innovation and discovery that has changed the way we think about the world.

The Department of Cell & Systems Biology promotes research and teaching in some of the most dynamic and vital areas of biological research. We facilitate the pursuit of cutting-edge research in the molecular life sciences and systems biology and embrace new methodologies and state-of-the-art technologies. Located in downtown Toronto and part of the University of Toronto, one of the world’s top research universities, our department is an ideal place to work, research and make a lasting impression in the field.

**Your opportunity**:
This is a one year term position covering a materinity leave.

**Your responsibilities will include**:

- Setting-up lab/workshop according to research and/or course schedules
- Preparing, assembling and setting-up material, equipment and supplies used in teaching and/or research labs
- Troubleshooting issues with lab equipment and/or workstations
- Ensuring the safe handling of specialized lab equipment and materials while operating within close proximity to others
- Serving as a technical resource on laboratory protocols, and experimental procedures and equipment

**Essential Qualifications**:

- Bachelor's Degree in Biology or Microbiology or acceptable combination of equivalent experience and education.
- Minimum two years of recent and relevant experience in providing technical and administrative support for Cell and Molecular Biology Teaching Labs, including Bioinformatics, PCR and electrophoresis, DNA extraction, Microscopy, plant transplantation and bacterial transformation
- Experience in preparing and assembling solutions, materials, supplies and equipment used for Physiology and Cell and Molecular Biology Teaching Labs
- Experience in employing a range of molecular techniques including: cloning, transformations, DNA/RNA extraction, ligation, PCR, plasmid preps, gel electrophoresis, yeast matings, plant seed sterilization, propagation and germination to run experiments
- Experience in employing a range of physiological techniques including: measurement of physiological parameters (e.g. Cardiorespiratory, EMG, neurophysiology), bioassays (e.g. Daphnia heart rate assay, C. elegans chemotaxis), associated techniques (e.g. fluorescence microscopy, PCR and gel electrophoresis) and Drosophila development
- Experience in preparing and aliquoting all the reagents and materials including PCR reagents, enzymes, DNA/RNA gels, stock solutions, bacterial plates, live organisms, etc.
- Experience in maintaining live animal colonies and growing live cultures used in teaching labs including Daphnia Magna,Planarians, American Cockroaches, C. elegans, Arabidospsis Thaliana, saccharomyces cerevisiae, Drosophila etc.
- Experience in operating and troubleshooting steam sterilizer machines (autoclaves)
- Experience in operating, maintaining, and trouble-shooting laboratory equipment including biosafety cabinet, pH meters, spectrometer, thermocycler, microscopes, pipettes, centrifuges, gel-running devices, computers and ancillary equipment
- Experience in maintaining departmental microscopesand other equipment and perform periodic functional tests including: pipettes, pH meter, spectrometer, gel electrophoresis devices, thermocycler, centrifuges and culturing equipment (incubator, cell culture hood), dissecting, compound, and fluorescent microscopes
- Experience in maintaining and performing function checks on computers and data acquisition instrumentation used in neurophysiology, ECG and other Physiology lab exercises
- Strong technical skills in all aspects mentioned above
- Excellent written and verbal communication skills, organizational ability, tact and diplomacy in dealing with others
- Demonstrated problem solving skill with the ability to proactively recognize issues
- Valid G driver’s license required for picking up and delivering animals, plants, microorganisms and supplies
- Ability to work evenings, weekends or holidays to suit the demands of teaching lab
- Experience i