Research Assistant/ Technician 3

3 weeks ago

Vancouver, Canada University of British Columbia Full time

Staff - Union

Job Category

CUPE 116

Job Profile

CUPE 116 Salaried - Research Assistant /Technician 3

Job Title

Research Assistant/ Technician 3

Department

Research | Wong

Compensation Range

$4,618.00 - $5,036.00 CAD Monthly

Posting End Date

June 3, 2023

Note: Applications will be accepted until 11:59 PM on the day prior to the Posting End Date above.

Job End Date

Jun 29, 2024

Job Summary

The focus of the laboratory is to investigate the structural maintenance of the human genome and the mechanisms of enzymes involved in these maintenance activities. Applicants must therefore have extensive knowledge and experience in various laboratory techniques including, but not limited to, cell culture (immortalized and transformed cell lines and primary cell lines), molecular biology (isolation of DNA and RNA, chromatin-immunopreciptation, RNA immunoprecipitation and quantitative PCR), imaging (epifluorescent and confocal microscopy of lived and fixed cells) and biochemistry (Western blotting and enzymatic assays). Experience with next-generation sequencing workflow and library preparation is a plus. The individual will be expected to perform experiments and data analysis in coordination with other lab members.

Our research routinely involves hazardous materials, including radioactive chemicals, organic solvents, acids and compressed gases. The laboratory is licensed to use radioactive material. All employees must complete the Biological, Chemical and Radiation Safety courses UBC offers. All cell culture work will be performed in a biological safety cabinet, and appropriate certification is mandatory.

Organizational Status

We have a fully operational lab with all the equipment necessary to complete our projects. Facilities are adequate to do the work performed. Our research routinely involves hazardous materials, including radioactive chemicals, organic solvents, acids and compressed gases. The laboratory is licensed to use radioactive material. All employees must complete the Chemical Safety and Radiation Protection courses offered by UBC. All cell culture work will be performed in a biological safety cabinet, and biological safety certificate is mandatory. Desk space is available in an area outside the laboratory.

Work Performed

Following well-established guidelines and procedures assists in carrying out experiments investigating the structural maintenance of the genome and how deficiencies in the maintenance activities impact human health.- Prepares reagents and buffers, manages cell cultures, and performs in vitro experiments. Generates and maintains primary as well as transformed human cell model in culture.- Extracts DNA and RNA from biological samples and uses molecular biology techniques including Southern and Northern blotting, PCR and RT-PCR to assess RNA expression and genotyping.- Perform chromatin immunoprecipitation and RNA immunoprecipitation experiments. Quantify with PCR- or NGS-based methods- Analyses, assess and interprets protein expression and activity using Western blotting, ELISA, and enzymatic assays.- Plans and performs immunofluorescence microscopy and/or immunohistochemistry on cultured cells and clinical specimens from human studies.- In collaboration with senior lab members, assists in optimizing protocols and troubleshooting of the lab.- Assists with training new students on cell culture work, molecular biology and biochemistry techniques, and use of instruments.- Analyzes, prepares and interprets experimental data using Prism GraphPad, Adobe Creative Suite and SigmaPlot. Presents data at laboratory meetings and assists in preparing manuscripts and grant proposals.- Performing other general laboratory-related duties as required.

Consequence of Error/Judgement

Failure to report problems could result in lost or unusable data from experiments resulting in a delay of project progress and requiring that the experiments be repeated.

Errors at any stage may result in loss of precious resources (biological samples) and a misinterpretation of the data. Repeated inaccuracies and errors of the same type are incompatible with the position.

Supervision Received

The incumbent will be supervised by the PI, but will need to communicate and work closely with graduate students in the laboratory. Once trained, the technician will work mainly independently following established protocols.

Supervision Given

Minimum Qualifications

Completion of a relevant technical program or a university degree in a relevant discipline and a minimum three years of related experience or an equivalent combination of education and experience.

Preferred Qualifications

MSc degree in pharmacology, biochemistry, molecular biology, or a related discipline desired. Preference will be given to those with experience in genetics or genomics research. Must have human cells and clinical specimen handling experience and be comfortable working with human materials. Previous documented experience with cell cultu

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