Research and Development Intern
7 days ago
ABOUT THE EMPLOYER:
Lallemand is a privately held Canadian company founded in the late 19th century, which develops, produces, and markets microorganisms for various markets. The administrative offices of the parent company are in Montreal, Canada. Today, Lallemand employs more than 4,500 people working in more than 45 countries on 5 continents.
Lallemand Health Solutions specializes in the production of probiotics intended to serve the agri-food, parapharmaceutical, and pharmaceutical industries. With its manufacturing processes adhering to the highest standards of quality, the company provides probiotic strains that help millions of people improve their health and well-being.
On top of its extensive portfolio of ready-to-use probiotics (Lacidofil, Probiokid, Probio'Stick, Protecflor), the Health Solutions branch also tailor-makes unique formulas to respond to clients' unique needs. This process employs the Harmonium, Lafti, or Rosell strains.
The Lallemand Health Solutions team enjoys a stellar reputation, as one of the only companies that is authorized to produce the S.boulardii strain. Whether in North America, Europe, or Asia, your next pro-fessional challenge awaits you at Lallemand
MAIN FUNCTIONS:
Reporting to the Preclinical Laboratory Manager, the intern will support the LHS research program by aiding in day-to-day activities of the lab, including but not limited to:
- General microbiology and bacterial culture.
- Cell culture models. This would include working with Caco-2, HT-29, and HMVII cells. The selected intern would assist in a number of projects ranging from adhesion assays, inflammation studies, and gut permeability/tight junction investigations, with the potential to have independent projects as well.
- Flow Cytometry for bacteria viability and cell quantification
- Western Blot Assays for Protein Quantification
- Use of liquid handling robots (Opentron OT-2 and FLEX)
- Method development and validations
- SOP and scientific reports writing
TECHNICAL SKILL & QUALIFICATIONS:
· Year 1 of Bachelor's degree in Life Sciences (biology, biochemistry, immunology, microbiology, etc), or engineering (Chemical, Biomedical, Bioengineering).
· Knowledge of good laboratory practices and Biological Safety 1 and 2 regulations.
· Laboratory Experience (University Laboratories included) - Mandatory
· Some background in microbiology - Asset
· Experience in Cell Culture work - Asset
· Good knowledge of English and French (oral and written).
· Ability to work within a team and independently.
· This position will be for 8 months
Term: January - August
________________________________________________________________________________________________________________________
Stagiaire recherche et développement (Laboratoire préclinique)
À PROPOS DE L'EMPLOYEUR:
Lallemand est une société canadienne privée fondée à la fin du XIXe siècle, qui développe, produit et commercialise des micro-organismes pour divers marchés. Les bureaux administratifs de la société mère sont situés à Montréal, au Canada. Aujourd'hui, Lallemand emploie plus de 4 500 personnes travaillant dans plus de 45 pays sur 5 continents.
Lallemand Solutions Santé se spécialise dans la production de probiotiques destinés aux industries agroalimentaires, parapharmaceutiques et pharmaceutiques. Avec ses processus de fabrication répondant aux meilleures normes de qualité, elle est en mesure de fournir des souches probiotiques qui contribuent à la santé et au bien-être de millions de personnes.
En plus d'offrir une gamme complète de formules probiotiques prêtes à l'emploi (Lacidofil, Probiokid, Probio'Stick, Protecflor), cette unité d'affaires conçoit des formulations sur mesure pour répondre aux besoins de ses clients, avec ses souches Harmonium, Lafti ou Rosell.
L'équipe de Lallemand Solutions Santé jouit d'une excellente réputation, étant l'une des seules à pouvoir produire la souche S.boulardii. Que ce soit en Amérique du Nord, en Europe ou en Asie, votre prochain défi s'y trouve peut-être
FONCTIONS PRINCIPALES:
Relevant du Gestionnaire de laboratoire préclinique, le stagiaire effectuera des tâches pour supporter les activités journalières dans le laboratoire préclinique. Ces tâches comprendront, mais ne seront pas nécessairement limité à:
· Microbioogie générale et culture bactériennes.
· Culture cellulaire. Cela inclurait le travail avec les cellules Caco-2, HT-29, HMVII et FaDu. Le stagiaire sélectionné participerait à un certain nombre de modèles allant des tests d'adhésion aux études sur l'inflammation et aux enquêtes sur la perméabilité intestinale et les jonctions serrées.
· Cytométrie en flux pour la viabilité des bactéries et la quantification cellulaire
· PCR quantitatif en temps réel (qPCR).
· Rédaction de protocoles normalisés et de rapports scientifiques.
Note : L'usage du masculin est employé sans discrimination et dans le but d'alléger le texte.
TECHNICAL SKILL & QUALIFICATIONS:
· Minimum 1 an de Baccalauréat en Sciences de la vie (biologie, biochimie, immunologie, microbiologie, etc), ou Génie Chimique.
· Connaissance des bonnes pratiques de laboratoire et des règles de sécurité en laboratoire (Niveau de sécurité biologique 1 et 2).
· Expérience de travail en laboratoire (incluant laboratoires universitaires) - Requis
· Connaissances en microbiologie - Atout
· Expérience dans le travail de culture cellulaire - Atout
· Bonne connaissance de l'anglais et du français (oral et écrit).
· Capable de bien travailler en équipe et de manière autonome.
· Ce poste sera d'une durée de 8 mois.
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