Germ Free Animal Care Technician, Physiology

4 weeks ago

Calgary, Canada The University of Calgary Full time

**Position Overview**

This position reports to the Scientific Director.

Germ Free Animal Care Technicians perform routine tasks of moderate complexity in animal husbandry for all areas of the IMC germ-free facility. Performance requirements must strictly follow standard operating procedures and safety standards. Some examples of duties include maintenance of isolators, isocage and experimental systems, preparation of sterile materials, and quality control.

This is a recurring part-time position with variable hours. Recurring part-time employees are hourly employees engaged to perform work on an irregular schedule.

**Position Description**

**Summary of Key Responsibilities** (job functions include but are not limited to):

- Under general supervision & direction of the Director of the IMC, develop and implement techniques and procedures within the guidelines of the research
- Standard laboratory technique, including
- Microbiological sampling techniques, testing and monitoring including making culture media, nucleic acid extraction and stains (Gram stains, etc), genotyping, PCR, qPCR, aerobic and anaerobic culturing, and other protocols as required
- Provides basic treatment for animals under direction of Germ-free facility director or manager, including injections, blood samples, antibiotics, euthonol, controlled drug administrations, etc
- Preparation and importation into isolators of materials required for experimental procedures
- Standard preparation for supplies, including
- Preparation of supply drums for autoclaving (food, bedding, water, supplies)
- Connecting of drums to isolators
- Keep all material stocks stocked and organized
- Support research staff with import of research materials
- Animal Husbandry, including
- Provide animal care for axenic, gnotobiotic and Level II pathogen animal models for all species of laboratory animals used in research, which includes feeding, watering and changing cages following strict germ-free and safety guidelines for prevention of exposure to microbes. Ensure adherence to the legal limit of maximal number of mice per cage according to cage type
- Monitors and assesses general animal health for axenic, gnotobiotic and Level II pathogen animal models, and reports problems to the Germ-free Director, manager or Supervisors
- Record keeping both manual and computer based for consistency in animal health, effective use of animal models and reporting to facility management
- Sterile import of supplies into isolators including sterilization of connecting sleeves
- Responsible for operation and up-keep of equipment such as but not limited to isolators, laminar flow cabinets, ventilated caging systems, autoclave, spray gun, hydrolic lifts, anaesthetic delivery systems and ventilators, biosafety cabinets, under safety guidelines. Advises supervisor of any equipment problems that require maintenance or repair
- Monitoring and maintaining of the climate parameters within the animal rooms
- Hygiene Responsibilities: Applies, monitors and ensures the hygiene concept
- Reporting responsibilities: Data entry and digital records for but not limited to animal husbandry, procedures and microbiology
- General, safety, housekeeping duties, including
- Custodial duties to maintain facility sanitation procedures (bottle washing, cage cleaning preparation, cleaning and decontamination of connection sleeves, cleaning of the rooms and floors, cleaning of showers, cleaning and autoclaving barrier shoes, preparation of barrier clothing and gear, cleaning of drums and sterilization trays, proper waste disposal, send and receive scrubs and gowns to and from washing service, receiving of goods and materials, etc)
- Assist team in development and maintenance of SOP's
- Work with chemicals safely and properly, e.g. dilutions, using appropriate containers and protective gear
- Responsible for keeping skills up-to-date and taking courses that will assist in personal and professional development
- Provide well developed and consistent technical support and animal care services to users of the Germ-free/Gnotobiotic/Level II pathogen facility and to ensure that proper procedures are followed at all times
- Provides orientation and training to users of the facility and ensures standard operating procedures and approved animal care protocols are adhered to
- Setting up new breeding pairs for researchers including maintaining clear records

**Qualifications / Requirements**:

- A minimum of a Bachelor Degree in Science or Animal Health Technology equivalent
- Two years technical experience working with animal research models and technical knowledge of microbiology would be considered an asset
- Work experience may include but is not limited to Animal research facility, Veterinary Clinic or other animal care organizations
- In-depth knowledge of theory, procedures and skills in areas of specialized husbandry and complex technical procedures
- Attention to detail is crucial in following procedures a

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