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Sessional Lecturer
4 months ago
Faculty / Division: Faculty of Medicine
Department: Molecular Genetics
Campus: St. George (downtown Toronto)
Job Posting: May 24, 2024
Job Closing: June 14, 11:59pm EST
Course number and title: MGY314H1: Principles of Genetic Analysis I Laboratory
Course description:
This is a laboratory course designed to introduce third- and fourth-year undergraduates to the principles and practice of genetic analysis in both prokaryotic and eukaryotic model organisms. Student teams carry out genetic crosses, mutant hunts, and phenotypic/genotypic characterization in bacteria, phage, and yeast mutants, and learn to analyze, interpret, and present the genetic data obtained. Students learn fundamental lab skills through experimentation (dilution streaking, cell culturing/counting, sterile technique, pipetting, serial dilutions, crossing strains etc. ) as well as standard genetic approaches including generating mutants, deducing gene function from phenotypic analysis, identifying genetic suppressors, characterizing mutant alleles (both phenotypically and genotypically), ordering genes in a genetic pathway (epistasis analysis) and generating genetic interaction profiles. Topics taught encompass the fundamental concepts of genetics: mutation, complementation, recombination, genetic suppression and regulation (epistasis)—and notably, how the tools of genetic analysis, interpret the results and present the data. While strong emphasis is placed on the “doing of proper genetics”—experimental design, the importance of controls, good lab habits (sterile technique, dilutions, organization and safe handling of all materials) are core learning objectives, as is the effective communication of scientific data through the production of high quality of data figures/tables and clear scientific writing. The model organisms used are Escherichia coli and Saccharomyces cerevisiae, both biosafety level 1 organisms.
Estimated TA support: 300 TA hours.
Estimated course enrolment: 60.
Class schedule: Thursdays (1:00 p.m. to 5:00 p.m.)
Sessional dates of appointment: September 1 to December 31, 2024
Salary: $9,457.90 for Sessional Lecturer I; $9,930.79 for Sessional Lecturer I Long Term;
$10,121.77 for Sessional Lecturer II; $10,362.76 for Sessional Lecturer III, inclusive of vacation pay Please note that should rates stipulated in the collective agreement vary from rates stated in this posting, the rates stated in the collective agreement shall prevail.
Qualifications:
The applicant must hold a completed or nearly completed PhD in the area of Microbiology or Molecular Genetics or other relevant field and is expected to obtain a valid biosafety certification for in-lab teaching (level 1 or higher). They should be experienced with the concepts and methods of genetic analysis in E. coli, phage and budding yeast and have taken upper year undergraduate/graduate courses in the subject area, or alternatively have thesis and/or teaching experience in classical genetic analysis/microbiology. The applicant should have demonstrated expertise in fundamental microbiological (E. coli) lab techniques and experimental genetic analysis techniques. Previous teaching experience in classical genetics is desirable but not required. Awareness of the GSA Genetics Learning Framework (genetics-gsa/education/genetics-learning-framework/) and demonstrated pedagogical expertise in implementing the framework in an upper year undergraduate setting is an asset.
Description of duties:
The MGY314H1F course is team taught in the fall semester (September to December) and consists of 12 weeks of genetics labs, broken up into two distinct parts (prokaryotic vs eukaryotic genetics). Each section of the course is independently overseen by a separate instructor and run in conjunction with the Department of Teaching Labs who are responsible for the materials required for the wet lab sessions (including the biological and chemical reagents) and provide additional behind the scenes support such as growing cultures, extra replica platings, incubating plates and removing to cold, monitoring experiments for contamination etc. The instructor meets weekly (~1H) with the teaching assistants and DTL prior to each in-lab session (usually Monday or Tuesday) at a mutually convenient time to review the learning objectives for the lab, provide TA training, assess the results from the previous week's experiments and answer any questions about the week's lab (may also be done by email). The sessional position is predominantly for the first 6 weeks of the course (focusing on bacterial genetics) and culminates in a 1H midterm test that is written and marked by the instructor. In addition, the instructor contributes questions/marking of the final exam in December, which normally counts for ~20% of the final exam.
The overall format of the course is that each week, the instructor posts a 30 to 60 min background lecture on-line (on Mondays) that the students must view prior to attending the wet lab session each Thursday from 1-5 pm. Student preparedness for the lab is assessed through existing on-line quizzes due before the wet lab starts. As the background lecture counts as part of the 4H weekly lab course time allotment, most students will complete the in-lab portion of the course within 3H, however some lab sessions will go a bit longer. The instructor is expected to attend all lab sessions, answer student questions, help prepare the lab beforehand, interface with DTL as well as contribute to the hiring, training, and supervision of the teaching assistants (who will be all new for 2024). The approximate time commitment is 10 hours per week. Some interactions with students may be required to be done online.
The course has been taught for several years. The successful applicant will have the support of the co-instructor/course coordinator who teaches the yeast section for the last 6 weeks of the course, manages the Quercus course page and takes the lead on hiring/training TAs and dealing with course administrative issues. As all of the TAs for MGY314 will be new for 2024, the instructor will be involved in recruiting, training and supporting the TAs in carrying out the bacterial genetics experiments. A laboratory manual, course slides, pre-recorded lectures, lab report templates (and marking notes), examples of prior assessments and other critical components are available and have already been used successfully. Reagents and equipment for each lab are overseen by the Dept of Teaching labs who may have questions about the materials and occasionally require help troubleshooting experimental issues. Minimal modification of course materials may be required to update them for use this year or in response to specific student challenges (for instance, we’ve had to reteach M1V1 to the covid cohorts as well as provide additional support in helping students learn to use basic graphing/table making software for their reports).
Application Procedure:
Applications should include a single PDF with the following components in this order: 1) the CUPE 3902 Unit 3 application form, available at:
uoft.me/CUPE-3902-Unit-3-Application-Form; 2) a cover letter; and 3) a c.v. that includes evidence of demonstrated expertise in classical and molecular genetic techniques in microbiology (notably phage/E.coli biology), as well as the name(s) of one or more qualified referees.
Please submit the applications electronically to:
Dr. Timothy Hughes
Professor and Chair, Department of Molecular Genetics
mogen.chairutoronto.ca
This job is posted in accordance with the CUPE 3902 Unit 3 Collective Agreement.
It is understood that some announcements of vacancies are tentative, pending final course determinations and enrolment. Should rates stipulated in the collective agreement vary from rates stated in this posting, the rates stated in the collective agreement shall prevail.
Preference in hiring is given to qualified individuals advanced to the rank of Sessional Lecturer II or Sessional Lecturer III in accordance with Article 14:12 of the CUPE 3902 Unit 3 collective agreement.
Please note: Undergraduate or graduate students and postdoctoral fellows of the University of Toronto are covered by the CUPE 3902 Unit 1 collective agreement rather than the Unit 3 collective agreement, and should not apply for positions posted under the Unit 3 collective agreement.
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